After defrag my average dropped to 54.4 and my burst increased to 170. I would've thought that defragging would help not hinder performance.
Also, before the defrag the line graph was fairly linear that tapered off gradually. After defrag the linear graph looked more like a heartbeat on an EKG.
On the second test were you sure your drive wasn't accessing any data? Perhaps a background process was using some drive I/O? Check perfmon to see if anything is running. Start->Run, type "perfmon". Click Add (or CTRL+I). Under Performance object, choose PhysicalDisk. Under the counters list choose Disk Transfers/sec. Click Close and on the bottom of perfmon click on Disk Transfers/sec. After that click the Highlight (CTRL+H) button. The highlight feature should highlight the the disk transfers with a big white line. Pay attention to the Average results.