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Difference in Expansion slots on asus m5a99fx pro r2.0

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Getting ready to add a gigabye geforce gtx 970 G1 gaming card to my motherboard. I am looking at the user guide for the motherboard and it looks like the expansion slots for slot 1 and 4 function the same way. I was going to just use port 1 but it would put the card close to eh 212 evo I have. I figured if both slots function the same, I would just move it down to give more room between the 2.

Labeled in User Manual as:Slot Number 1 - "PCIe 2.0 x16_1 (dual at x16/x16 mode)"
Slot Number 4 - " "PCIe 2.0 x16_3 (dual at x16/x16 mode)"

moboslots.jpg 175k .jpg file

Pulled straight from Asus page for mobo with guide on page: (version E8043)